ANALISIS VARIASI GENETIK AMORPHOPHALLUS MUELLERI BLUME DARI BERBAGAI POPULASI DI JAWA TIMUR BERDASARKAN SEKUEN INTRON trnL
DOI:
https://doi.org/10.32556/floribunda.v4i6.2013.102Keywords:
Amorphophallus muelleri, genetic variability, sequences, trnL intron, East JawaAbstract
The objective of this study is to determine the genetic variability of porang atdifferent populations based on analysis of trnL intron sequences. Research was conducted in East Jawa, which is porang population at Jember (Mayang: MY), Malang (Lawang: LW, Kalipare: KP), Blitar (Brongkos: BL), Nganjuk (KPH tritik: TR), Madiun (Klangon: KL, KPH Saradan: MD), Bojonegoro (Klino: KN). Exploration conducted in 8 populations to take samples of young leaves as many as three sheets at two different individuals of each population. Then extract the DNA, and amplify the trnL intron using universal primers 'c' and 'd', then were purified and sequenced. The results of sequencing were analyzed the genetic variation and relationship based on Maximum Parsimony tree by MEGA5 software. Sequencing of 16 individuals porang, produce 13 individuals which have a clear chart chromatography which is resulting in the proper sequence and can be used in subsequent analyzes. The sequences showed the differences in nucleotide base composition of each individual. Six region is also known as conserved region, 52 sequences nucleotide variability, and 8 deletion. The phylogeny tree showed four clade Major. Porang were clustered randomly, not based on the population to which the individual is taken. Thus, the origin of porang at each study site cannot be identified based on the geographical analysis.Downloads
Published
2016-02-27
Issue
Section
Articles
License
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeThe Effect of Open Access).